A kind of fibroin nanoparticles taking and its preparation method and application

A kind of fibroin nanoparticles taking and its preparation method and application

  • CN 106,474,482 A
  • Filed: 09/28/2016
  • Published: 03/08/2017
  • Est. Priority Date: 09/28/2016
  • Status: Active Grant
First Claim
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1. a kind of preparation method of fibroin nanoparticles taking, it is characterised in that comprise the following steps:

  • S1. the silk fibroin protein solution of purifying is taken, the mass concentration of silk fibroin protein solution is adjusted to 0.01-0.6%, in liquid nitrogen ringIt is refrigerated in border and is fully cured, obtains fibroin albumen Frozen Body;

    S2. fibroin albumen Frozen Body is carried out freeze-drying process, obtains fibroin nanoparticles taking powder;

    S3. fibroin nanoparticles taking powder is added in alcoholic solution of the mass concentration for 50-100%, carries out ultrasonic wavelength-division successivelyScattered, centrifugal treating, obtains the mixed liquor of lower leaf, collects supernatant liquid;

    S4., supernatant liquid is carried out centrifugal treating, washing and ultrasonic wave dispersion successively, the fibroin albumen for obtaining favorable dispersibility is receivedRice grain.

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