The method and its equipment of a kind of continuous chromatography

The method and its equipment of a kind of continuous chromatography

CN
  • CN 107,185,273 A
  • Filed: 06/15/2017
  • Published: 09/22/2017
  • Est. Priority Date: 06/15/2017
  • Status: Active Application
First Claim
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1. the method and its equipment of a kind of continuous chromatography, it is characterised in that:

  • It is described to be equipped for multicolumn continuous chromatography system, the systemSystem is divided into Liang great Qu:

    Crude separation area and smart Disengagement zone;

    The crude separation area is made up of five subprovinces:

    Loading area, Qu Qianza areas, goThe areas of Hou Za 1, sample elution area, the areas of Qu Houza 2, are defined as I, II, III, IV and V area successively;

    The smart Disengagement zone is by two AsiasDistrict'"'"'s groups into:

    Smart Disengagement zone and sample elution area, are defined as VI and VII area successively;

    The subprovince is by 1~n(

    n =1、

    2、

    3、

    4、

    5、

    6Or 7)

    Branch Coupled columns are constituted, and are connected between chromatographic column and chromatographic column by pipeline or/and valve interface;

    Methods described is:

    It is manyComponent sample is in I area'"'"'s Pulsed Sampling;

    After single injected sampling, through valve transfer, Ith area end post carry is the first post in IIth area, in the flowing of IIth areaMutually elution is miscellaneous before removing(

    Target compound is eluted to and just penetrates IIth area)



    Through valve transfer, IIth area end post carry is that IVth area is firstTarget compound, is eluted to just completely into the last post in IVth area by post in IV area'"'"'s mobile phase;

    Removed in III and V area'"'"'s mobile phase elutionIt is miscellaneous afterwards;

    Through valve transfer, IVth area end post carry is the first post in VIth area, and sample is finely separated in VIth area(

    Mobile phase is by targetedCompound, which is eluted to, just penetrates VIth area)



    Through valve transfer, VIth area end post carry is the first post in VIIth area, in the mobile phase elution segmentation of VIIth areaCollect target compound and containing a small amount of rear miscellaneous target compound;

    Methods described passes through valve transfer, the start and stop of pump or/and streamThe intelligent controls such as velocity modulation control are realized.

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