A kind of method of ultra-violet analysis vitamin B6 content in crude product

A kind of method of ultra-violet analysis vitamin B6 content in crude product

  • CN 107,314,982 A
  • Filed: 06/30/2017
  • Published: 11/03/2017
  • Est. Priority Date: 06/30/2017
  • Status: Active Application
First Claim
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1. a kind of method of ultra-violet analysis vitamin B6 content in crude product, it is characterised in that this method comprises the following steps:

  • A, reagent preparation test solution:

    Prepare concentration and be used as reagent test solution for 0.1mol/l HCI solution.B, required instrument and equipment:

    Required instrument and equipment includes ultraviolet specrophotometer and balance.C, preparation solution:

    1), reference substance stock solution;

    Precision weighs vitamin B6 CP standard items 0.050g, is placed in 50mL brown volumetric flask, uses0.1mol/L HCI solution dissolving in step A, the concentration for making vitamin B6 CP standard items is 1000ug/ml, is settled to scale,Shake up;

    Condition of storage:

    It is kept in dark place in the refrigerator for being placed into 2-8 DEG C.2) sample solution, is prepared:

    Crude product vitamin B6 is dried:

    Crude product vitamin B6 is dried to constant weight at 105 DEG C;

    3) sample solution, is prepared:

    Precision weighs dried sample 0.050g, is placed in 50mL brown volumetric flasks, uses 0.1mol/L HCI solution dissolves, and is settled to scale, shakes up;

    Precision measures 5.0mL, is placed in 50mL brown volumetric flasks, is diluted to scale with 0.1mol/L HCI solution, shakes up;

    Sample solution at least prepares 2 parts.4) reference substance solution, is prepared;

    Precision measures reference substance stock solution 5.0mL, is placed in 50mL brown volumetric flasks, uses0.1mol/L HCI solution is diluted to scale, shakes up.D, measure:

    Precision measures 0.1mol/L HCI solution 5.0mL, is placed in 50mL brown volumetric flasks, with 0.1mol/mL HCISolution makees blank, and the absorbance of determination sample solution and reference substance solution is distinguished at 291nm wavelength, and sample is calculated using external standard methodProduct content.E, cubage:

    Calculation formula is as follows:

    Wherein:

    The absorbance of A samples --- sample solution;

    A is right --- the absorbance of reference substance solution;

    C is to storage --- and reference substance stock solution concentration, unit is ug/ml;

    W samples --- dried sample sample weighting amount, unit is g.

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