A kind of method of active ingredient in measure ageratum oral liquid

A kind of method of active ingredient in measure ageratum oral liquid

  • CN 107,389,821 A
  • Filed: 07/25/2017
  • Published: 11/24/2017
  • Est. Priority Date: 07/25/2017
  • Status: Active Application
First Claim
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1. A kind of 1. method for determining active ingredient in ageratum oral liquid, it is characterised in that comprise the following steps:

  • 1) preparation of standard reference material solution:

    Take aurantiamarin, magnolol and honokiol standard reference material appropriate, it is accurately weighed, add methanol to be configured to every 1mL and contain orange peelGlycosides, magnolol and honokiol are respectively 0.05~0.30mg mixed solution;

    0.2~0.5 μ

    m of membrane filtration, it is to be measured;

    2) preparation of need testing solution:

    Take ageratum oral liquid stoste or stoste is diluted 2-5 times with 60~100% methanol/ethanol;

    0.2~0.5 μ

    m of filterMembrane filtration, it is to be measured;

    3) determined using high performance liquid chromatograph:

    Step

         1) is put into high performance liquid chromatograph with the standard liquid and need testing solution

         2) prepared, using two benches gradientElution method determines, and chromatographic condition is as follows:

    Chromatographic column is C18 chromatographic columns, and column temperature is 25~40 DEG C, and mobile phase is methanol-acetonitrile-water, and flow velocity is 0.2~2mL/min,Sample size is 2~20 μ

    L (determining actual sample size, flow velocity according to liquid chromatograph and chromatographic column specification);

    Gradient collapse program is pressedCarried out according to following table:

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