A kind of screening technique of the feeding probiotics of anti-pig salmonella infection

A kind of screening technique of the feeding probiotics of anti-pig salmonella infection

  • CN 107,893,110 A
  • Filed: 12/29/2017
  • Published: 04/10/2018
  • Est. Priority Date: 12/29/2017
  • Status: Active Application
First Claim
Patent Images

1. a kind of screening technique of the feeding probiotics of anti-pig salmonella infection, it is characterised in that comprise the following steps:

  • Step S10, the morbidity piglet of salmonella infection and disease-resistant piglet are selected, corresponding collection morbidity piglet and disease-resistant pigletIntestinal contents sample;

    Step S20, the intestinal microflora genome in the intestinal contents sample of extraction morbidity piglet and disease-resistant piglet, andHigh-flux sequence is carried out to intestinal microflora;

    Step S30, metagenomics comparative analysis is carried out to the sequencing data of high-flux sequence;

    Step S40, according to the result of metagenomics comparative analysis, the difference in morbidity piglet and disease-resistant intestine of young pigs flora is comparedDifferent flora, core flora and dominant microflora, and analyze from the difference flora being only present in disease-resistant intestine of young pigs thereinDominant bacteria and core bacterium, the purpose bacterial strain of probiotics to be screened is determined according to dominant bacteria and with the analysis result of core bacterium, thenAccording to the bacterium colony characteristic of purpose bacterial strain, strain culturing is carried out using Selective agar medium, that is, filters out anti-pig salmonella infectionProbiotics.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×