Novel antigens and methods therefor

Novel antigens and methods therefor

  • CN 1,080,304 C
  • Filed: 07/25/1990
  • Issued: 03/06/2002
  • Est. Priority Date: 07/25/1989
  • Status: Active Grant
First Claim
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1. proteic method of modified L of producing hepatitis B virus, described proteic aminoacid sequence is made up of the ad of hepatitis B virus or the proteic partial amino-acid series of L of ay hypotype, it is characterized in that this proteic aminoacid sequence or by the proteic residue 12-52 of (a) described L, the back is residue 133-145, and the back is that residue 175-400 forms;

  • Perhaps by the proteic residue 12 of (b) described L, the back is residue 14-52, and the back is residue 133-145, and the back is that residue 175-400 forms;

    Described method comprises;

    (i) preparation contains encoding said proteins, and can operate the expression vector of the dna sequence dna that links to each other with regulating and controlling sequence;

    (ii) transform the host cell that is selected from yeast cell with this carrier;

    (iii) in suitable medium, cultivate this host cell, separate described albumen from cell pyrolysis liquid or in the extract of described substratum.

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