Process for the efficient production of 7-ADCA VIA 3-(carboxyethylthio)propionyl-7-ADCA

Process for the efficient production of 7-ADCA VIA 3-(carboxyethylthio)propionyl-7-ADCA

  • CN 1,087,780 C
  • Filed: 07/29/1994
  • Issued: 07/17/2002
  • Est. Priority Date: 07/30/1993
  • Status: Active Grant
First Claim
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1. one kind prepares the method that 7-amino removes acetate Cephalosporanic acid (7-ADCA), comprising:

  • A) the filamentous fungus expression signal transcribe with translational control under, with ring enlargement enzyme gene transformation Penicllium chrysogenum bacterial strain;

    B) described bacterial strain ferments in substratum, and in described substratum, add 3,3 '"'"'-thio-2 acid or its salt or ester, they are suitable for producing 3-(carboxyethylthio) propionyl-6-amino-penicillanic acid, and (3-(carboxyethylthio) propionyl-6-APA), latter'"'"'s ring expansion on the spot forms 3-(carboxyethylthio) propionyl-7-ADCA;

    C) from fermented liquid, reclaim 3-(carboxyethylthio) propionyl-7-ADCA;

    D) with described 3-(carboxyethylthio) propionyl-7-ADCA deacylation;

    AndE) reclaim crystalline 7-ADCA.

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