Process for the efficient production of 7-ADCA via 2-(carboxyethylthio)acetyl-7-ADCA and 3-(carboxymethylthio)propionyl-7-ADCA

Process for the efficient production of 7-ADCA via 2-(carboxyethylthio)acetyl-7-ADCA and 3-(carboxymethylthio)propionyl-7-ADCA

  • CN 1,103,814 C
  • Filed: 07/29/1994
  • Issued: 03/26/2003
  • Est. Priority Date: 07/30/1993
  • Status: Active Grant
First Claim
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1. one kind prepares the method that 7-amino removes acetate Cephalosporanic acid (7-ADCA), comprising:

  • A) the filamentous fungus expression signal transcribe with translational control under, with ring enlargement enzyme gene transformation Penicllium chrysogenum bacterial strain;

    B) described bacterial strain ferments in substratum, and in described substratum, add 3 '"'"'-carboxy-methyl mercapto-group propionic acid or its salt or ester, they be suitable for producing 2-(carboxyethylthio) acetyl-and 3-(carboxy-methyl mercapto-group) propionyl-6-amino-penicillanic acid (2-(carboxyethylthio) acetyl-6-APA and 3-(carboxy-methyl mercapto-group) propionyl-6-APA), described 6-APA derivative ring expansion on the spot form 2-(carboxyethylthio) acetyl-7-ADCA and 3-(carboxy-methyl mercapto-group) propionyl-7-ADCA;

    C) from fermented liquid, reclaim 2-(carboxyethylthio) acetyl-7-ADCA and 3-(carboxy-methyl mercapto-group) propionyl-7-ADCA;

    D) with described 2-(carboxyethylthio) acetyl-7-ADCA and 3-(carboxy-methyl mercapto-group) propionyl-7-ADCA deacylation;

    AndE) reclaim crystalline 7-ADCA.

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