Subtilisin BPN' variants with decreased adsorption and increased hydrolysis

Subtilisin BPN' variants with decreased adsorption and increased hydrolysis

  • CN 1,134,728 A
  • Filed: 08/31/1994
  • Published: 10/30/1996
  • Est. Priority Date: 09/15/1993
  • Status: Abandoned Application
First Claim
Patent Images

1. BPN '"'"' the variant that contains the wild-type amino acid sequence, it is characterized in that the one or more locational amino acid in the 199th, 200,201,202,203,204,205,206,207,208,209,210,211,212,213,214,215,216,218,219 or 220 of wild-type aminoacid sequence is replaced, whereinA.199 Wei alternative amino acid is Cys, Ala, His, Thr, Pro, Gly, Gln, Ash, Ser, Asp or Glu;

  • B.200 Wei alternative amino acid is His, Thr, Pro, Gly, Gln, Asn, Ser, Asp or Glu;

    C.201 Wei alternative amino acid is Gly, Gln, Asn, Ser, Asp or Glu;

    D.202 Wei alternative amino acid is Pro, Gln, Asn, Ser, Asp or Glu;

    E.203 Wei alternative amino acid is Met, Cys, His, Pro, Gly, Gln, Asn, Ser, Asp or Glu;

    F.204 Wei alternative amino acid is Glu;

    G.205 Wei alternative amino acid is Leu, Met, Cys, Ala, His, Thr, Pro, Gly, Gln, Asn, Ser, Asp or Glu;

    H.206 Wei alternative amino acid is Pro, Asn or Ser;

    I.207 Wei alternative amino acid is Asp or Glu;

    J.208 Wei alternative amino acid is Pro, Gly, Gln, Asn, Ser, Asp or Glu;

    K.209 Wei alternative amino acid is Ile, Val, Met, Cys, Ala, His, Thr, Pro, Gly, Gln, Asn, Ser, Asp or Glu;

    1.210 the alternative amino acid of position is Gly, Gln, Asn, Ser, Asp or Glu;

    M.211 Wei alternative amino acid is Ala, Pro, Gln, Asn, Ser, Asp or Glu;

    N.212 Wei alternative amino acid is Gln, Ser, Asp or Glu;

    O.213 Wei alternative amino acid is Trp, Phe, Tyr, Leu, Ile, Val, Met, Cys, Ala, His, Pro, Gly, Gln, Asn, Ser, Asp or Glu;

    P.214 Wei alternative amino acid is Phe, Leu, Ile, Val, Met, Cys, Ala, His, Pro, Gly, Gln, Asn, Asp or Glu;

    Q.215 Wei alternative amino acid is Thr, Pro, Gln, Asn, Ser, Asp or Glu;

    R.216 Wei alternative amino acid is His, Thr, Pro, Gly, Gln, Asn, Ser, Asp or Glu;

    S.218 Wei alternative amino acid is Glu;

    T.219 Wei alternative amino acid is Pro, Gln, Asn, Ser, Asp or Glu;

    AndU.220 Wei alternative amino acid is Pro, Gly, Gln, Asn, Asp or Glu;

    It is characterized in that BPN '"'"' variant compares it and reduced the sorption of insoluble substrate and increased hydrolytic action to insoluble substrate with wild-type subtilisin BPN '"'"'.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×