Method of preparing monoclonal antibody, monoclonal antibody, pharmaceutical composition and diagnostic reagent

Method of preparing monoclonal antibody, monoclonal antibody, pharmaceutical composition and diagnostic reagent

  • CN 1,188,115 A
  • Filed: 12/05/1997
  • Published: 07/22/1998
  • Est. Priority Date: 12/06/1996
  • Status: Abandoned Application
First Claim
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1. prepare the method for anti-cell surface antigen monoclonal antibodies, may further comprise the stepsto comprise the material injection Mammals of cell-surface antigens, described material is selected from i) whole cell and ii) by handling the membrane portions that whole cell obtains;

  • 2) from described mammiferous spleen, separate the cell part that contains the B-cell;

    3) described cell part in specific B-cell by obtaining in the following steps enriching step 2 to described cell-surface antigens, comprise;

    with contacting of this cell part and the cell relevant in conjunction with carrier substance with described whole cell, this cell in conjunction with the carrier related substances lack described cell-surface antigens and from the enrichment that is ready to use in next step not in conjunction with separation and combination the cell part that contains the B-cell to relevant cell in conjunction with the B-cell on the carrier substance;

    4) cell that contains the B-cell that the step in front of enrichment is obtained partly carries out limited dilution and then carries out clonal expansion;

    5) screening B-cell clone also infinitely breeds the described B-cell clone that filters out with the small-scale integration technology;

    With6) screening and clone can produce the hybridoma of specificity in conjunction with described cell-surface antigens antibody, then separate the part that comprises monoclonal antibody from the supernatant liquor of described hybridoma.

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