Gene encoding endo-beta-N-acetylglucosaminidase A

Gene encoding endo-beta-N-acetylglucosaminidase A

  • CN 1,216,996 C
  • Filed: 10/26/1996
  • Issued: 08/31/2005
  • Est. Priority Date: 10/27/1995
  • Status: Active Grant
First Claim
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1. in coding has-and β

  • -active separated DNA of N-acetyl glucosamine acid enzyme A, this separated DNA is selected from;

    (a) has the DNA of SEQ ID NO;

    2 nucleotide sequence;

    (b) has the DNA of the nucleotide sequence of coding SEQ ID NO;

    1 aminoacid sequence;

    (c) in the solution that contains 5 * SSC, 0.5%SDS, 100 μ

    g/ml salmon sperm DNAs and 5 * Denhardt '"'"' s, 60 ℃

    down can with the dna sequence dna of (a) hybridization, wherein 1 * SSC=8.77g NaCl and 4.41g Trisodium Citrate are dissolved in the solution of gained in 1 premium on currency, 5 * Dehardt '"'"' s solution contains bovine serum albumin, polyvinylpyrrolidone and Ficoll, each 0.1% concentration.

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