Culture of long chain unsaturated fatty acid by heterotrophic marine microalgal

Culture of long chain unsaturated fatty acid by heterotrophic marine microalgal

  • CN 1,218,035 C
  • Filed: 12/11/2000
  • Issued: 09/07/2005
  • Est. Priority Date: 12/11/2000
  • Status: Active Grant
First Claim
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1. , a kind of substratum comprises carbon source, nitrogenous source, inorganic salt, and wherein said carbon source is selected from waste molasses, Semen Maydis powder, sucrose, fructose, lactose, glucose, Zulkovsky starch carbonic acid gas or its mixture;

  • Described nitrogenous source is selected from organic nitrogen compound, inorganic nitrogen compound or its mixture, and described inorganic nitrogen compound comprises ammonium nitrate, ammonium sulfate, urea or its mixture;

    Described organic nitrogen compound comprises proline(Pro), arginine, tyrosine, aspartic acid, peptone, L-glutamic acid or its mixture;

    Described inorganic salt are selected from following a kind of material at least;

    sodium salt, sylvite, magnesium salts, carbonate molysite, manganese salt, cobalt salt, molybdate, zinc salt, boride, bromide and composition thereof;

    Wherein said carbon source concentration is 10-200g/L;

    Used nitrogen concentration is the 1/2-1/10 of carbon source concentration in the substratum;

    Described inorganic salt concentration is a standard with the sodium salt, is 0.01-12ppm, and the pH value of substratum is 3.0-12.0.

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