In vitro method for construction of DNA library

In vitro method for construction of DNA library

  • CN 1,249,784 A
  • Filed: 03/18/1998
  • Published: 04/05/2000
  • Est. Priority Date: 03/18/1997
  • Status: Abandoned Application
First Claim
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1. be shifted from a series of different initial single or double-stranded parental DNA templates and a kind of method in primer structure reorganization homology polynucleotide library by inductive template in the external polynucleotide that use polysaccharase are synthetic, wherein A. is by synthetic primer or the polynucleotide of extending of following methodA) sex change of parental generation double-stranded DNA template is produced single-stranded template,B) with above-mentioned primer and single stranded DNA template annealing,C) use above-mentioned polysaccharase by the above-mentioned primer of initial synthetic extension,D) cause that synthetic suppresses, andE) with double-stranded sex change, separate the primer that extends from template, B. lures the displacement of anomalous mode plate by following methodA) separating new synthetic strand from template extends primer and uses the above-mentioned extension primer that produces (A)As primer and template repeating step A.b) to A.e), orB) repeating step A.b) to A.e), C. in proper number round-robin steps A. and B.a), A. and B.b), or its combination back termination said process, and D. selectively uses the specific primer polynucleotide that amplification produces in Standard PC R reaction with the interested homology polynucleotide of selective amplification.

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