Technology of preparing rat nerve growth factor using organic solvent virus deactivation method

Technology of preparing rat nerve growth factor using organic solvent virus deactivation method

  • CN 1,302,009 C
  • Filed: 09/05/2005
  • Issued: 02/28/2007
  • Est. Priority Date: 09/05/2005
  • Status: Active Grant
First Claim
Patent Images

1. , with the technology of preparing rat nerve growth factor using organic solvent virus deactivation method,, gets the homogenate supernatant liquor with the mouse submandibular gland tissue homogenate;

  • Then that the homogenate supernatant liquor is centrifugal, remove post precipitation, last ion exchange chromatography I, collect stream and wear liquid, stream is worn liquid and is carried out acidifying and dissociate, centrifugal, get the acidolysis supernatant liquor, it is characterized in that with water for injection dilution acidolysis supernatant liquor to contained protein concentration be 2.7~

    3.3mg/ml, to wherein adding organic solvent, the organic solvent final concentration is 2.00~

    2.35% (m/v), and constant temperature is 4~

    6 hours in 24~

    37 ℃

    of water-baths, go up ion exchange chromatography II then, collect the target protein peak, must collect liquid, collect ion exchange chromatography III on the liquid, promptly get NGF stoste, add vehicle N.F,USP MANNITOL and stablizer albumin again, after Sterile Filtration, packing, freeze-drying gets goodsThe medium of ion exchange chromatography III is a cationite,Organic solvent is a tributyl phosphate.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×