Amplifying and detecting target nucleic acids using post amplification incubation step

Amplifying and detecting target nucleic acids using post amplification incubation step

  • CN 1,309,838 C
  • Filed: 03/11/1997
  • Issued: 04/11/2007
  • Est. Priority Date: 03/11/1996
  • Status: Active Grant
First Claim
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1. an amplification and measure the method for target nucleic acid, it comprises:

  • (a) sample that suspection is contained described target nucleic acid contacts with a kind of stable on heating amplification enzyme with at least two kinds of oligonucleotide, and the part of wherein said at least two kinds of oligonucleotide and described target nucleic acid is complementary, and used condition makes described target nucleic acid amplification;

    (b) make the target nucleic acid sex change of amplification to form single-chain nucleic acid;

    (c) as the culturing step after the amplification, cultivate described sample between 1 second and 30 minutes and between 95 ℃ and

    120 ℃

    , make thermotolerance amplification enzyme deactivation;

    (d) measure the target nucleic acid that whether has described amplification;

    Wherein the inactivation of thermotolerance amplification enzyme does not require and opens reaction vessel.

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