Fungal transcriptional activator useful in methods for producing polyptides

Fungal transcriptional activator useful in methods for producing polyptides

  • CN 1,329,667 A
  • Filed: 10/05/1999
  • Published: 01/02/2002
  • Est. Priority Date: 10/05/1998
  • Status: Active Grant
First Claim
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1. isolated nucleic acid sequences, its a kind of polypeptide of encoding with transcriptional activation activity, it is selected from:

  • (a) nucleotide sequence of at least 70% identity is arranged with the nucleotide sequence of SEQ ID NO;

    1;

    (b) nucleotide sequence of coding one peptide species, the aminoacid sequence of described amino acid sequence of polypeptide and SEQ ID NO;

    2 has at least 50% identity.(c) under low rigorous degree condition with (i) nucleotide sequence of SEQ ID NO;

    1, the perhaps (ii) nucleotide sequence of its complementary strand hybridization, wherein will hang down rigorous degree conditional definition in 42 ℃

    5 * SSPE, 0.3%SDS, 200mg/ml shear and the salmon sperm dna of sex change and 25% methane amide in prehybridization and hybridization, and wash conditions is defined as in 50 ℃

    of washings 30 minutes in 2 * SSC, 0.2%SDS;

    (d) (a) and (b) or allele variant (c);

    (e) (a) and (b), (c) or subsequence (d), wherein a kind of polypeptide fragment of this subsequence coding with transcriptional activation activity;

    And(f) (a) and (b), (c) or subsequence (d), wherein a kind of polypeptide of this subsequence coding with aminoacid sequence of SEQ ID NO;

    3.

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