Method and compositions for directed clonning and subclonning using homologous recombination

Method and compositions for directed clonning and subclonning using homologous recombination

  • CN 1,373,803 A
  • Filed: 07/10/2000
  • Published: 10/09/2002
  • Est. Priority Date: 07/09/1999
  • Status: Abandoned Application
First Claim
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1. method that double-stranded target DNA is imported carrier, the bacterium that comprises the functional recombinase of culture expression, described bacterial cell comprises (a) target DNA, contain first double-stranded terminal and second double-stranded end, and (b) carrier DNA, on the carrier DNA chain, contain in the following sequence:

  • (i) (ii) replication orgin of first double-stranded homology arm, and (iii) second double-stranded homology arm, like this, first homology arm sequence of carrier DNA chain and first end sequence homology of target dna strand, second homology arm sequence of carrier DNA chain and second end sequence homology of target dna strand, like this, target DNA can insert in the carrier DNA between homology arm.

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