Single-stranded oligodeoxynucleotide mutation vectors

Single-stranded oligodeoxynucleotide mutation vectors

  • CN 1,384,759 B
  • Filed: 08/25/2000
  • Issued: 05/22/2013
  • Est. Priority Date: 08/27/1999
  • Status: Active Grant
First Claim
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1. be used for obtaining the method for mammalian cell, this mammalian cell contains predetermined heredity on target gene changes, and the method comprises:

  • (a) provide mammalian cell colony in culture medium;

    (b) following compositions is added culture medium;

    And(c) cell with this predetermined heredity variation in colony is identified, wherein, described mammalian cell is not embryonic stem cellDescribed compositions is used for producing predetermined heredity and changes on the target gene of mammals or birds cell, said composition contains;

    (a) strand oligodeoxynucleotide, this oligodeoxynucleotide has 3 '"'"' terminal nucleotide, 5 '"'"' terminal nucleotide, have at least 31 Deoxydization nucleotides and be no more than 59 Deoxydization nucleotides, and has the sequence that contains at least two zones, at least 8, each zone Deoxydization nucleotide, these zones are identical with at least two zones on target gene respectively, few 24 nucleotide are grown in these zones altogether, and these zones are separated by at least one nucleotide in both sequence in the sequence of this target gene or in the sequence of this oligodeoxynucleotide or simultaneously, so that the sequence of this oligodeoxynucleotide is not identical with the sequence of target gene, and(b) macromolecular carrier, these carriers are selected from(i) the lipid vesicle of core water, wherein this core water contains this strand oligodeoxynucleotide,(ii) lipid nanometer spheroid, this spheroid contains the Lipophilic salts of this strand oligodeoxynucleotide, and(iii) polycation, it has 500 dalton to the mean molecule quantity of 1.3Md, and wherein this polycation forms salt with this strand oligodeoxynucleotide.

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