Process for preparing bioactive peptide-silk fibroin peptide

Process for preparing bioactive peptide-silk fibroin peptide

  • CN 1,392,265 A
  • Filed: 07/23/2002
  • Published: 01/22/2003
  • Est. Priority Date: 07/23/2002
  • Status: Active Application
First Claim
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1. the preparation method of a silk peptide is characterized in that utilizing the molten silk of enzymatic hydrolysis liquid, removes the CaCl of enzymolysis solution middle and high concentration again with nanofiltration 2Salt, concrete steps are:

  • be raw material with leftover bits and pieces silk cocoon or useless silk

         1), with the Na of 0.3-1.0% (w/v) 2CO 3Solution is concise,

         2) with the CaCl of 20-60% (w/w) 2Solution is 50-140 ℃

    of dissolving,

         3) molten silk liquid is at pH2.5-12.0, carry out enzymatic hydrolysis reaction under 30-80 ℃

    ,

         4) enzyme that goes out after control hydrolysis degree DH is 5-25%,

         5) reaction finishes, centrifugal, filter, getting filtrate is enzymolysis solution,

         6) enzymolysis solution carries out the nanofiltration desalination with nanofiltration membrane, continuous thin up feed liquid in demineralising process,

         7) feed liquid after the desalination carries out spraying drying after concentrating and promptly gets product silk peptide powder.

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