Production of recombinant blood clotting factors in human cell lines

Production of recombinant blood clotting factors in human cell lines

  • CN 1,454,257 A
  • Filed: 03/21/2001
  • Published: 11/05/2003
  • Est. Priority Date: 03/22/2000
  • Status: Abandoned Application
First Claim
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1. method that is used to produce recombinant human blood coagulation factor, described method comprises:

  • (a) cultivating a kind of immortalization human cell is, at least a virus transcription activator of described clone stably express and carrying has the carrier with the functional promotor that is connected of dna sequence dna of the described thrombin of coding, and condition is that described promotor is not the viral promotors that is stimulated by described at least a virus transcription activator;

    With(b) from liquid nutrient medium, separate described thrombin.

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