Process for obtaining insulin and insulin derivatives having correctly bonded crystine bridges

Process for obtaining insulin and insulin derivatives having correctly bonded crystine bridges

  • CN 1,483,831 A
  • Filed: 08/17/1998
  • Published: 03/24/2004
  • Est. Priority Date: 08/18/1997
  • Status: Abandoned Application
First Claim
Patent Images

1. the precursor acquisition from Regular Insulin or insulin derivates has the method for the Regular Insulin or the insulin derivates of correctly bonded cystine linkage, described precursor is at halfcystine or cysteine hydrochloride and folding process of experience in the presence of the liquid auxiliary agent, after this folding process, obtain Regular Insulin or insulin derivates from described precursor in the following way:

  • adopt trypsinase or tryptase and the optional protaminase that adopts in addition to carry out enzymatic lysis, then on polymeric adsorbent, carry out purifying;

    This method comprises implements following steps successively to finish described folding process;

    (a) aqeous suspension with the precursor of Regular Insulin or insulin derivates mixes with a certain amount of halfcystine or cysteine hydrochloride, and this amount causes the cysteine residues of the SH residue/precursor of 1~

    15 halfcystine or cysteine hydrochloride;

    (b) the pH value about 8~

    about 15~

    about 55 ℃

    of about 11.5, temperature under, the precursor suspension that will contain halfcystine or cysteine hydrochloride join concentration be 4~

    9M in the liquid compounding agent solution, the gained mixture was kept under described temperature about 10~

    60 minutes;

    With(c) the pH value about 8~

    about 5~

    about 30 ℃

    of about 11.5, temperature under, mixture is joined in a certain amount of water, make the concentration dilution of halfcystine in the mixture or cysteine hydrochloride to about 1~

    5mM, from concentration dilution to the 0.2~

    1.0M of liquid auxiliary agent.

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