Chemical amplification electrochemical detecting method for affinity reaction and agent case thereof

Chemical amplification electrochemical detecting method for affinity reaction and agent case thereof

  • CN 1,504,746 A
  • Filed: 12/03/2002
  • Published: 06/16/2004
  • Est. Priority Date: 12/03/2002
  • Status: Active Application
First Claim
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1. , chemistry amplifies electrochemical detection method, may further comprise the steps:

  • 1) provides the reactant that can be on oxidizing electrode combines and/or react with analyzed goal analysis thing;

    2) the described reactant that is provided in the sample that may contain the goal analysis thing and the step

         1) contacts3) the described reactant that is provided in the sample that may contain the goal analysis thing and the step

         1) contacts.Under appropraite condition, described analyte combines with described reactant.Described reactant, analyte or additional reactant or additional analysis thing or analyte analog be in the electrochemical activity labeled molecule covalent bond of going back ortho states and be connected.Labeled molecule is oxidized on electrode;

    4) with the reductive agent that electrochemical reaction can not take place on electrode the electrochemical activity molecule of described affinant is reverted to and go back ortho states;

    5) the described electrochemical activity molecule that is reduced repeats to participate in step

         3) and

         4) described oxidation-reduction reaction, the electrochemical signals that produce to amplify;

    6) estimate the electrochemical signals that amplifies, determine the existence of goal analysis thing in sample and/or the amount of goal analysis thing.

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