Alzheimer's disease diagnosis based on mitogen-activated protein kinase phosphorylation

Alzheimer's disease diagnosis based on mitogen-activated protein kinase phosphorylation

  • CN 1,549,721 A
  • Filed: 02/27/2002
  • Published: 11/24/2004
  • Est. Priority Date: 02/27/2001
  • Status: Active Grant
First Claim
Patent Images

1. the method for an alzheimer'"'"'s disease diagnosis in the experimenter, described method comprises:

  • (a) this experimenter is measured the foundation level of a kind of index protein phosphorylation in its cell;

    (b) experimenter'"'"'s cell is contacted with a kind of activator, described activator and index are proteic to be selected according to being, the phosphorylation process that index albumen is activated in this experimenter'"'"'s cell, compare with the phosphorylation process that is activated in the contrast experimenter cell of taking from no Alzheimer, when handling with activator, in one period scheduled time after beginning to handle, both can cause different reactions to activator to this;

    (c) after the contact beginning, the proteic phosphorylation level that is activated of testing index in described experimenter'"'"'s cell;

    And(d) ratio of the basic phosphorylation level of the phosphorylation level that is activated of calculating mensuration in step (c) and step (a);

    And(e) with the ratio that calculates in the step (d) with determine in the past, in the described scheduled time, compare with ratio from the phosphorylation process that is activated of non-Alzheimer raji cell assay Raji from known Alzheimer cell;

    It is characterized in that, if the ratio of this calculating and past do not have significant difference to the determined ratio of described known Alzheimer cell, diagnosis is exactly positive, and/or, if the ratio of this calculating and past do not have significant difference to the determined ratio of described non-Alzheimer cell, then diagnosis is exactly negative.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×