Specific strain for degrading organic waste water of medicine production and its construction method

Specific strain for degrading organic waste water of medicine production and its construction method

  • CN 1,594,539 A
  • Filed: 06/30/2004
  • Published: 03/16/2005
  • Est. Priority Date: 06/30/2004
  • Status: Active Application
First Claim
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1. , degraded pharmacy waste water specific strain, it is characterized in that with Parental plant 1White-rot fungi (Phanerochaetechrysosporium), Parental plant 2Native bacterium XZ1 (Bacillus), Parental plant 3Merge yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) eukaryotic cell protoplastis and prokaryotic cell prokaryocyte protoplastis preparation back.First preparation protoplastis, and collect, obtain after the buffer solution for cleaning through centrifugal back, carry out the secondary fusion then.Merge for the first time:

  • equivalent Parental plant 1The protoplastis of white-rot fungi with Parental plant 2The protoplastis of native bacterium XZ1 cell mixes and at polyoxyethylene glycol, CaCl 2, sucrose preparation induced liquid in merge, through centrifugal back collection fusion product;

    Merge for the second time;

    the bacterium colony of the equivalent that obtained last time with Parental plant 3The protoplastis of brewing yeast cell mixes, at polyoxyethylene glycol, CaCl 2, sucrose preparation induced liquid in merge, after collecting fusion product after centrifugal, containing sucrose high osmotic buffer eccentric cleaning, separate application on three kinds of solid differential mediums of two anti-substratum of SIM1 Streptomycin sulphate Sm substratum, SIM2 nystatin Nt substratum, S IM3 Streptomycin sulphate Sm+ nystatin Nt, the bacterium colony that the while can grow on three kinds of substratum is that the three parents preservation of transboundary merging number is the bacterium colony of CGMCC.1087.

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