Macrophage in vitro inducing culture method

Macrophage in vitro inducing culture method

  • CN 1,609,199 A
  • Filed: 11/17/2004
  • Published: 04/27/2005
  • Est. Priority Date: 11/17/2004
  • Status: Active Application
First Claim
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1. a macrophages in vitro method for inducing and cultivating is characterized in that the mononuclearcell that will be separated to PRMI1640 substratum furnishing 2 * 10 from blood, tissue or seroperitoneum 6Behind individual cell/ml, place 37 ℃

  • , 5%CO 2In the incubator, when the pH value of substratum reduce to 4 or below, all culturing cell volume-diminished, endochylema endoparticle increase, refractive power deformation weak after, continue to be cultured to that small amounts of cells occurs that cell volume becomes greatly gradually, the endochylema endoparticle reduces, refractive power deformation is strong, begin to change on a small quantity every day liquid this moment, when treating that these cells enter quick division growth, change liquid more according to a conventional method, going down to posterity gets final product.

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