Solid phase antibody direct competition carbofuran enzyme joint immune abzorption analysis technology and its kit

Solid phase antibody direct competition carbofuran enzyme joint immune abzorption analysis technology and its kit

  • CN 1,624,479 A
  • Filed: 10/20/2004
  • Published: 06/08/2005
  • Est. Priority Date: 10/20/2004
  • Status: Active Application
First Claim
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1. , a kind of insolubilized antibody direct competitive enzyme-linked immunosorbent analytical technique and kit thereof that is used for the carbofuran fast detecting, it is characterized in that with Be haptens, with the synthetic artificial antigen of protein covalent coupling, with the antibody of artificial antigen immune animal preparation to carbofuran tool specificity affinity, with the horseradish peroxidase-labeled haptens, with described antibody sandwich polystyrene micropore plate, add testing sample or carbofuran standard specimen and enzyme and mark haptenic mixed liquor, carbofuran, the competitive combination takes place with the antibody that is coated on micropore surface in enzyme mark haptens, educt is removed in washing, the substrate and the developer that add enzyme, the intensity of enzyme-catalytic chromogenic reaction is marked the haptens amount with the enzyme on being combined in antibody and is directly proportional, be inversely proportional to the content of carbofuran in sample or the standard specimen, set up carbofuran direct competitive enzyme-linked immunosorbent analytical technique in view of the above, use this technology, related reagent and material are set in box, prepare immunity detection reagent, be used for the fast detecting of agricultural product and the residual carbofuran of environment.

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