Immune chromatography with fluorescent rare earth nanometer particle as marker and detecting testing paper strip

Immune chromatography with fluorescent rare earth nanometer particle as marker and detecting testing paper strip

  • CN 1,645,146 A
  • Filed: 02/03/2005
  • Published: 07/27/2005
  • Est. Priority Date: 02/03/2005
  • Status: Active Application
First Claim
Patent Images

1. with the serve as a mark immune chromatography method of thing of fluorescent rare earth nanometer particle, it is characterized in that the steps include:

  • 1) directly be fixed on chromatographic film on by physical adsorption way capture antigen or antibody;

    To can not directly being fixed on the less antigen of molecular weight on the chromatographic film, earlier that micromolecule antigen and carrier protein is crosslinked, the mode by physisorption is fixed on the chromatographic film again;

    2) sample pad one side in chromatographic film adds sample liquid and labelled antibody, form immune complex through immune response, said labelled antibody refers to monoclonal that combines with determined antigen or the polyclonal antibody with the fluorescent rare earth complex compound nanoparticle label, and said immune complex forms through immunochromatography and detects band and control band;

    3) detection band and the control band that forms detected.

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