Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores

Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores

  • CN 1,675,377 B
  • Filed: 06/20/2003
  • Issued: 10/03/2012
  • Est. Priority Date: 06/20/2002
  • Status: Active Grant
First Claim
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1. measuring method comprises:

  • The sample that contains herbicide-tolerant polynucleotide under a cloud is provided;

    Provide and herbicide-tolerant polynucleotide generation electrostatic interaction, and when exciting, can transfer the energy to signal and transmit chromophoric polycation, water-soluble, that obtain light, luminous many chromophoric grouies, said many chromophoric grouies comprise conjugated polymers;

    Provide single stranded form and with herbicide-tolerant polynucleotide complementary sensing PNAG3, this sensing PNA and signal transmit chromophoric group and put together;

    Make sample under given conditions with solution in sensing PNA contact with many chromophoric grouies, wherein said specified conditions are meant, if there is herbicide-tolerant polynucleotide, sensing PNA can with the condition of said herbicide-tolerant polynucleotide hybridization;

    But apply the light source of excitation multi look group;

    AndDetect and whether transmit chromophoric group emission light by signal,Wherein when many chromophoric grouies excite, the light quantity of being transmitted the chromophoric group emission by signal is greater than the light quantity that when directly excitation signal transmits chromophoric group, obtains,And wherein said signal transmits chromophoric group and is selected from optical dye, semiconductor nanocrystal, lanthanide chelate and green fluorescent protein.

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