Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores

Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores

  • CN 1,694,967 B
  • Filed: 08/26/2003
  • Issued: 12/15/2010
  • Est. Priority Date: 08/26/2002
  • Status: Active Grant
First Claim
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1. the testing method of herbicide-tolerant polynucleotide in the working sample comprises:

  • Provide and suspect the sample that contains herbicide-tolerant polynucleotide;

    Be provided at and transmission ofenergy can be given the many chromophoric grouies of the chromophoric polycation of signal after exciting;

    Provide and herbicide-tolerant polynucleotide complementary negatively charged ion transmitter polynucleotide, described transmitter polynucleotide are conjugated on the signal chromophoric group, wherein said transmitter polynucleotide can interact with many chromophoric grouies and can produce emission light by the signal chromophoric group after many chromophoric grouies excite, and compare with the situation that the noncomplementation polynucleotide exist wherein that many chromophoric grouies excite the back to produce more substantial emission light by the signal chromophoric group under the situation that herbicide-tolerant polynucleotide exists;

    If the transmitter polynucleotide can with the condition of the herbicide-tolerant polynucleotide hybridization that exists under, sample is contacted in solution with many chromophoric grouies with the transmitter polynucleotide;

    With the many chromophoric grouies of light source activation;

    WithDetection is not only strengthened under the situation that sample exists by the emission of signal chromophoric group.

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