Determination method of creatnine content and reagent box for diagnosing creatnine

Determination method of creatnine content and reagent box for diagnosing creatnine

  • CN 1,757,755 A
  • Filed: 10/10/2004
  • Published: 04/12/2006
  • Est. Priority Date: 10/10/2004
  • Status: Active Grant
First Claim
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1. creatinine content determination method, step is as follows:

  • 1), with sample and the reagent mix of mainly forming by 2-oxoglutaric acid ester, reducibility coenzyme, Creatinine deiminase, glutamate dehydrogenase, make it to take place the reaction of following principle;

    Creatinine+water+H +Creatinine deiminaseN-methyl hydantoin+ammonium ion Ammonium ion+2-oxoglutaric acid ester+reduced coenzyme Glutamate dehydrogenaseL-glutamic acid + coenzyme2), detect the end reaction thing in the speed that predominant wavelength 340nm absorbancy descends, calculate the size of creatinine content.

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