• CN 1,774,504 A
  • Filed: 02/09/2004
  • Published: 05/17/2006
  • Est. Priority Date: 02/07/2003
  • Status: Active Application
First Claim
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1. the proteolytic enzyme of peptide enzyme family S2A and/or peptide enzyme family S1E, itsI) under pH7 and 25 ℃

  • , in being supplemented with the analysis buffer of 0.1%K-Sorbate, and there is the Cu of 1mM 2+In time, cultivated after 164 hours, and the residual activity of measurement has at least 0.80 residual activity with respect to the activity of cultivation after 0 hour;

    And/or Ii) with respect to there not being Cu 2+Contrast, have the Cu of 1mM 2+The time, have at least 0.66 relative reactivity;

    I wherein) and activity measurement ii) be pH7.0 and 25 ℃

    , in analysis buffer, on substrate Suc-AAPF-pNA, measure;

    AndWherein for i) observed value, proteolytic enzyme has at least 90% the purity of determining through SDS-PAGE.

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