Artificial hybridization and breeding method for male Paralichthys dentatus and female summer flounder

Artificial hybridization and breeding method for male Paralichthys dentatus and female summer flounder

CN
  • CN 1,843,100 A
  • Filed: 04/08/2005
  • Published: 10/11/2006
  • Est. Priority Date: 04/08/2005
  • Status: Active Application
First Claim
Patent Images

1. , male summer flounder and female lefteye flounder artificial hybridization and seedling-cultivating method, it is characterized in that with the summer flounder as male parent and lefteye flounder as female parent, by the parent is carried out fortification and environment conditioning, impel its sexual gland ripe synchronously, obtain mature sperm and ovum, carry out the artificial hybridization insemination, comprise the steps:

  • 1) respectively to the summer flounder and lefteye flounder carry out fortification and environment conditioning, impel its sexual gland ripe synchronously, obtain mature sperm and ovum;

    2) get summer flounder mature sperm and lefteye flounder mature egg and carry out artificial insemination;

    Describedly get summer flounder mature sperm and the lefteye flounder mature egg carries out artificial insemination, be meant summer flounder seminal fluid is splashed in the lefteye flounder ovum, add sea-water activated sperm, constantly stirred 2~

    3 minutes, adding seawater and left standstill 5~

    7 minutes, filter with 80 mesh sieve tulles, is 15~

    19 ℃

    of sand filtration seawater flushing fertilized eggs 3~

    5 times with temperature, fertilized egg is put into graduated cylinder leave standstill, get the come-up ovum and hatch;

    3) artificial regulatory incubating oosperm;

    Described artificial regulatory incubating oosperm is meant 16~

    20 ℃

    of water temperatures, salinity 30~

    32 ‰

    , and pH8.0~

    8.2, aeration quantity is 10~

    20L/m per hour 3Hatch under the condition;

    4) artificial regulatory son, postlarva are cultivated;

    Described artificial regulatory son, postlarva are cultivated, and are meant 16~

    20 ℃

    of water temperatures, salinity 30~

    32 ‰

    , and pH8.0~

    8.2, aeration quantity is changed from small to big, and the envirment factor that quantity of exchanged water increases is gradually carried out artificial regulatory;

    5) open-mouthed bait and seed rearing phase series bait throws something and feeds;

    Throwing something and feeding of described open-mouthed bait and seed rearing phase series bait is meant that open-mouthed bait is the wheel animalcule or the shellfish young;

    Training seedling whole process using wheel animalcule →

    artemia nauplii →

    mixed bait, fry grows during throw something and feed in early days wheel animalcule and the artemia nauplii, adds marine chlorella, and chlorella density keeps 80-120 ten thousand cell/ml;

    The program of throwing something and feeding;

    the 3-35 days wheel animalcules of throwing something and feeding in hatching back, keep 8-10/ml by the density of throwing something and feeding 20-40/ml in the water body;

    The 16-60 days artemia naupliis of throwing something and feeding in hatching back, the density of throwing something and feeding 10-20/ml, keeping density in the water is 3-5/ml;

    Hatched back 26 days, and began to tame the mixed bait of ingesting.

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