Detecting and typing method for hoof and mouth disease virus RT-PCR

Detecting and typing method for hoof and mouth disease virus RT-PCR

  • CN 1,858,246 A
  • Filed: 03/22/2006
  • Published: 11/08/2006
  • Est. Priority Date: 03/22/2006
  • Status: Active Application
First Claim
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1. , a kind of foot and mouth disease virus RT-PCR detects and classifying method, it is characterized in that:

  • design P1/P2 and two pairs of primers of S1/S2, wherein primer P1/P2 is positioned at 7567-7590bp and 8000-8023bp, conservative at 7 serotype foot-and-mouth disease virus gene group camber, the PCR product is 457bp, is mainly the 3D Gene Partial;

    Primer S1/S2 is positioned at 3266-3285bp and 3917-3936bp, PCR product size is 670bp, be mainly the small portion of VP1 gene major part and 2A gene, this segmental phase homologous serotype homology is all more than 80%, the different serotypes homology is all less than 69%, thereby determines the serotype of the strain that detects according to PCR product nucleotide homology.

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