Method for detecting id marks related to tumor in blood serum from patient of oesophagus cancer

Method for detecting id marks related to tumor in blood serum from patient of oesophagus cancer

  • CN 1,873,410 B
  • Filed: 06/16/2005
  • Issued: 11/30/2011
  • Est. Priority Date: 06/16/2005
  • Status: Active Grant
First Claim
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1. method that detects tumour correlating markings thing in the cancer of esophagi human serum is characterized in that:

  • A, gather cancer of esophagi human peripheral sample, centrifugal and separate upper serum under 4 ℃

    of temperature in back 2-3 hour of exsomatizing, obtain tumour serum;

    B, gather the healthy human peripheral blood sample, centrifugal and separate upper serum under 4 ℃

    of temperature in back 2-3 hour of exsomatizing, obtain control serum;

    The cell line total protein of C, extraction in vitro culture;

    D, precipitate tumour autoantigen in the described cell line total protein, separate obtaining tumour antigen-antibody complex with described patient'"'"'s tumor sero-immunity;

    Simultaneously, with the described cell line total protein of described control serum immunoprecipitation, obtain non-specific immune complex;

    E, use the SDS-PAGE electrophoresis, press the described tumour antigen-antibody complex of molecular weight size separation, obtain swimming lane 1 albumen;

    Simultaneously, use the SDS-PAGE electrophoresis,, obtain swimming lane 2 albumen according to the described nonspecific immunity compound of molecular weight size separation;

    F, described swimming lane 1 albumen of contrast and swimming lane 2 albumen, downcut tomour specific expressed proteins band, this protein band is carried out glue endotrypsin enzymolysis, digestion, the extracting of peptide section, the separation of hybrid peptide section liquid chromatography and the online ion trap mass spectrometry albumen of electro-spray ionization coupling attribute identify, obtain tumour correlating markings thing.

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