Methods for determining nucleotide sequence information

Methods for determining nucleotide sequence information

  • CN 1,898,398 A
  • Filed: 12/24/2004
  • Published: 01/17/2007
  • Est. Priority Date: 12/29/2003
  • Status: Active Application
First Claim
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1. method of measuring the nucleotide sequence of target nucleic acid comprises:

  • A) this nucleic acid or its fragment are contacted with a group capture oligonucleotide probe, comprise the probe-target duplex nucleic acid of strand external part with formation, described a group capture oligonucleotide probe is attached on the series of points position of matrix;

    B) described probe-target duplex nucleic acid is contacted with a group Raman active oligonucleotide probe, combine with described strand external part to allow described Raman microprobe, wherein each Raman active oligonucleotide probe produces unique raman signatures;

    C) use the Raman active oligonucleotide probe of Raman spectroscopy detection in conjunction with template nucleic acid;

    WithE) identify the some position of each captive Raman active oligonucleotide probe, thereby measure the nucleotide sequence of described target nucleic acid.

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