The frozen lactic acid bacteria culture of individual particle

The frozen lactic acid bacteria culture of individual particle

  • CN 1,922,305 B
  • Filed: 02/24/2005
  • Issued: 11/25/2015
  • Est. Priority Date: 02/24/2004
  • Status: Active Grant
First Claim
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1. additive compound is in the purposes of the pellet-frozen lactic culture of a kind of commercially available related packaging of preparation, and described packaging has the frozen matter of at least heavy 50g, and wherein frozen matter exists with the form of individual particle, containing at least 10 9the bacterium alive of individual colony-forming unit/gram frozen matter, and comprise the additive compound accounting for frozen matter 10%w/w amount, wherein said additive compound is cyclodextrin, maltose alcohol, fish glue or glue spraying, it is further characterized in that:

  • when using the additive compound accounting for frozen matter 10%w/w amount, this compound can increase the Tm '"'"' of frozen lactic acid bacteria culture extremely higher than-46 DEG C, described frozen lactic acid bacteria culture wherein not containing additive compound has the Tm '"'"' value of-70 DEG C to-46 DEG C, Tm '"'"' is that ice melts beginning temperature, it passes through determine with dsc method, and wherein said frozen lactic acid bacteria culture is characterized in that, when at being housed in-46 DEG C during 7-14 days, the individual particle of described frozen cultures does not stick together, therefore individual particle is remained, measured by following test at this;

    frozen cultures individual particle pellet-frozen in liquid nitrogen, 100 individual particles are poured in culture dish, thus define loose, the thin layer of individual particle, this laminar character is, one or more particles that most of particle is adjacent directly contact, at-46 DEG C, place 7-14 days and check particle whether still loose or particle whether condense into block or stick together, the judging criterion wherein remaining individual particle for the individual particle of frozen cultures is that 80 at least 100 individual particles remain loose individual particle.

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