DNA molecule identifying method for bluish dogbane and poacynum hendersonii

DNA molecule identifying method for bluish dogbane and poacynum hendersonii

  • CN 1,944,672 B
  • Filed: 08/09/2006
  • Issued: 01/18/2012
  • Est. Priority Date: 08/09/2006
  • Status: Active Grant
First Claim
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1. the dna molecular discrimination method of kendir and largeleaf poacynum leaf;

  • It is characterized in that this method extracts the genomic dna of kendir and largeleaf poacynum leaf testing sample respectively;

    With the genomic dna that extracts as template, utilize Auele Specific Primer under the situation of two different annealing temperature, to carry out the two-wheeled pcr amplification;

    Agarose gel electrophoresis carries out the discriminating of kendir and largeleaf poacynum leaf according to the different electrophoresis behaviors of two-wheeled PCR product;

    Wherein;

    The upstream primer of Auele Specific Primer is SEQ ID No.1, and downstream primer is SEQ ID No.2;

    Carry out two-wheeled PCR reaction to same sample, two-wheeled PCR reaction only exists different in annealing temperature, be respectively 50~

    62 ℃ and

    62.7 ℃

    , and all the other steps are identical;

    The judgement criteria of kendir and largeleaf poacynum leaf being differentiated according to the different electrophoresis behaviors of two-wheeled PCR product is second to take turns the PCR product and use conventional agarose gel electrophoresis;

    If have no band in the electrophoretogram;

    Then institute'"'"'s test sample article are kendir;

    If at the 220bp place clear wall scroll band is arranged, then institute'"'"'s test sample article are largeleaf poacynum leaf.

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