Mutant receptors and their use in a nuclear receptor-based inducible gene expression system

Mutant receptors and their use in a nuclear receptor-based inducible gene expression system

  • CN 1,964,622 A
  • Filed: 05/02/2005
  • Published: 05/16/2007
  • Est. Priority Date: 04/30/2004
  • Status: Active Application
First Claim
Patent Images

1. gene expression regulating system comprises expression casette, and described expression casette can be expressed in host cell, and described expression casette comprises the polynucleotides of coded polypeptide, and described polypeptide comprises:

  • I) transactivation domain;

    Ii) discern the DNA binding structural domain of response element, the gene that described response element and its expression are to be conditioned is connected; and

    Iii) H organizes the nuclear receptor ligands binding structural domain, it comprises at least one sudden change, and the position of described sudden change is selected from following amino acid residue place being equal to or being similar to;

    a) at least one in 48 of SEQ ID NO;

    1,51,52,54,92,95,96,109,110,119,120,125,128,132,219,223,234 and 238;

    B) 96 of SEQ ID NO;

    1 and 119;

    C) 110 of SEQ ID NO;

    1 and 128;

    D) 52 of SEQ ID NO;

    1 and 110;

    E) 107 of SEQ ID NO;

    1,110 and 127;

    F) 52 of SEQ ID NO;

    1,107 and 127;

    And g) 107,127 and 259 of SEQ ID NO;

    1, or their any combination.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×