Instrument and system for rapid microogranism identification and antimicrobial agent susceptibility testing
First Claim
1. A system for automated microorganism identification and antibiotic susceptibility testing comprising:
- a reagent cartridge comprising a plurality of wells;
a reagent stage wherein the reagent stage comprises an annular shape defining an interior opening, wherein the reagent stage is configured to rotate in a first plane and wherein the reagent stage is configured to receive the reagent cartridge;
a cassette comprising a plurality of microfluidic sample channels structured to receive samples, each of the plurality of microfluidic sample channels comprising an inlet port configured to receive a pipette tip;
a cassette stage located within the interior opening of the reagent stage, wherein the cassette stage is configured to rotate in a second plane parallel to the first plane and to move laterally in the second plane, and wherein the cassette stage is configured to receive the cassette;
a pipettor assembly configured to move a plurality of reagents between the plurality of wells of the reagent cartridge and the inlet ports of each of the plurality of microfluidic sample channels;
an optical detection system configured to obtain darkfield and fluorescence photomicrographs of one or more microorganisms contained in the plurality of microfluidic sample channels; and
a controller configured to direct operation of the system and process microorganism information derived from photomicrographs obtained by the optical detection system.
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Accused Products
Abstract
A system for automated microorganism identification and antibiotic susceptibility testing comprising a reagent cartridge, a reagent stage, a cassette, a cassette, stage, a pipettor assembly, an optical detection system, and a controller is disclosed. The system is designed to dynamically adjust motor idle torque to control heat load and employs a fast focus process for determining the true focus position of an individual microorganism. The system also may quantify the relative abundance of viable microorganisms in a sample using dynamic dilution, and facilitate growth of microorganisms in customized media for rapid, accurate antimicrobial susceptibility testing.
404 Citations
29 Claims
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1. A system for automated microorganism identification and antibiotic susceptibility testing comprising:
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a reagent cartridge comprising a plurality of wells; a reagent stage wherein the reagent stage comprises an annular shape defining an interior opening, wherein the reagent stage is configured to rotate in a first plane and wherein the reagent stage is configured to receive the reagent cartridge; a cassette comprising a plurality of microfluidic sample channels structured to receive samples, each of the plurality of microfluidic sample channels comprising an inlet port configured to receive a pipette tip; a cassette stage located within the interior opening of the reagent stage, wherein the cassette stage is configured to rotate in a second plane parallel to the first plane and to move laterally in the second plane, and wherein the cassette stage is configured to receive the cassette; a pipettor assembly configured to move a plurality of reagents between the plurality of wells of the reagent cartridge and the inlet ports of each of the plurality of microfluidic sample channels; an optical detection system configured to obtain darkfield and fluorescence photomicrographs of one or more microorganisms contained in the plurality of microfluidic sample channels; and a controller configured to direct operation of the system and process microorganism information derived from photomicrographs obtained by the optical detection system. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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Specification