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Methods and devices for quantitation of glycated protein

  • US 20030068830A1
  • Filed: 01/31/2002
  • Published: 04/10/2003
  • Est. Priority Date: 01/31/2001
  • Status: Active Grant
First Claim
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1. A method of quantitation of glycated protein in a sample which comprises:

  • (a) contacting a solid support matrix which comprises a negatively charged group and a hydroxyboryl compound and which has a measurement area, with an aliquot of biological sample sufficient to cover said measurement area;

    (b) contacting said solid support matrix with an aliquot of a first buffer sufficient to rinse off unbound protein wherein said first buffer has a pH selected to allow both glycated and non-glycated protein to be bound to said solid support matrix;

    (c) quantitating protein bound to said measurement area using measurement of a selected property of said protein to give a first bound protein reading;

    (d) contacting said solid support matrix with an aliquot of a second buffer sufficient to rinse off unbound protein wherein said second buffer has a pH selected to allow glycated protein to be bound to said solid support matrix but where non-glycated protein is not substantially bound to said solid support matrix;

    (e) quantitating protein bound to said measurement area using measurement of the property measured in step (c) to give a second bound protein reading; and

    (f) calculating percentage of glycated protein using said first and second bound protein readings.

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