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Multiplexed methylation detection methods

  • US 20030170684A1
  • Filed: 12/03/2002
  • Published: 09/11/2003
  • Est. Priority Date: 02/07/2000
  • Status: Active Grant
First Claim
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1. A method for multiplex detection of methylation of target nucleic acids comprising:

  • a. providing a first population of target nucleic acids labeled with a purification tag;

    b. cleaving said first population of target nucleic acids with an enzyme, whereby said enzyme discriminately cleaves at methylated target sequences forming a second population of cleaved target sequences;

    c. immobilizing said first and second populations by said purification tag; and

    d. detecting the presence of said first population comprising non-cleaved target nucleic acid whereby the presence of said first population comprising non-cleaved target nucleic acid indicates the presence of methylated target nucleic acids.

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