Data frame selection for cytometer analysis
First Claim
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1. A method for detecting an optimum core stream in a channel, comprising:
- at a starting time, providing a sample of particles to form a core stream that flows in a channel;
directing light at the particles of the core stream flowing in the channel;
detecting light scattered by the particles in the core stream during a series of time intervals;
converting the light scattered by the particles detected at a forward angle light scattering (FALS) angle and a small angle light scattering (SALS) angle, into FALS versus SALS data for each interval of time of the series of time intervals after the starting time;
analyzing the FALS versus SALS data for each interval of time; and
selecting a time duration from the series of time intervals where the FALS versus SALS data exhibit an approximately certain count and grouping of data.
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Abstract
A system for detecting when a core stream in a channel of a flow device is adequate for sufficient data accession. The system may determine the time period from the beginning of a sample run in a channel of a flow device to when the core stream is adequate. Data of detected FALS versus SALS light scattered by particles of the core stream may be plotted in data frames during a series of time intervals. The quality of the grouping and count rate of the data may indicate which frame and corresponding time interval reveal an adequate core stream.
80 Citations
42 Claims
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1. A method for detecting an optimum core stream in a channel, comprising:
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at a starting time, providing a sample of particles to form a core stream that flows in a channel;
directing light at the particles of the core stream flowing in the channel;
detecting light scattered by the particles in the core stream during a series of time intervals;
converting the light scattered by the particles detected at a forward angle light scattering (FALS) angle and a small angle light scattering (SALS) angle, into FALS versus SALS data for each interval of time of the series of time intervals after the starting time;
analyzing the FALS versus SALS data for each interval of time; and
selecting a time duration from the series of time intervals where the FALS versus SALS data exhibit an approximately certain count and grouping of data. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. An optimization detection system comprising:
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a processor connected to a FALS and SALS detector mechanism situated proximate to a light source arrangement having a flow channel situated between the FALS and SALS detector mechanism and the light source arrangement; and
wherein the processor is for recording signals from the FALS and SALS detector mechanism. - View Dependent Claims (16, 17, 18, 19, 20)
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21. A method for detecting an optimum core stream in a flow channel, comprising:
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beginning an insertion of particles to form a core stream sheathed by a fluid to flow in a channel;
directing light at the particles in the channel;
detecting and converting light scattered by the particles into electrical signals; and
processing the electrical signals in accordance with an algorithm for a determination of when the core stream is optimum for data taking. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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35. Means for detecting an optimum core stream in a channel of a flow device, comprising:
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means for providing particles to form a core stream that flows in a channel starting at time zero;
means for directing light at the particles of the core stream flowing in the channel; and
means for determining an optimum core stream from light scattered by the particles. - View Dependent Claims (36, 37, 38, 39, 40, 41)
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42. A data frame selection system comprising:
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a processor connected to a light scatter detector mechanism proximate to a light source arrangement having a flow channel situated between the light source arrangement and the light scatter detector mechanism; and
wherein the processor comprises an analyzer for identifying an optimum flow of a core stream in the flow channel sufficient for data taking.
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Specification