Electrodes linked via oligomers to nucleic acids

• US 20060099631A1
• Filed: 12/06/2005
• Published: 05/11/2006
• Est. Priority Date: 11/05/1996
• Status: Active Grant

• Current Assignee: Roche Molecular Systems Inc (Roche Holding AG)
• Original Assignee: Clinical Micro Sensors Inc. (Roche Holding AG)
• Inventors: Meade, Thomas J., Yu, Changjun, Kayyem, Jon F., Gozin, Michael, O'Conner, Stephen D.
• Granted Patent: US 7,381,533 B2
• Time in Patent Office: Days
• US Class Current: 435/6.11
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors

Abstract

The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electron transfer moieties and electrodes to produce a new class of biomaterials, and to methods of making and using them.

Claims

1-46. -46. (canceled)

47. A composition comprising:

a) an electrode comprising;

i) a monolayer; and

ii) a first nucleic acid covalently attached to said electrode via a spacer; and

b) a second nucleic acid comprising at least one electron transfer moiety (ETM) covalently attached to said second nucleic acid.

48. A composition according to claim 47 wherein said spacer is a conductive oligomer.

49. A compositions according to claim 47 wherein said spacer is an insulator.

50. A composition according to claim 47 wherein said spacer comprises alkyl chains.

51. A composition according to claim 47 wherein said second nucleic acid comprises a plurality of ETMs.

52. A composition according to claim 47 wherein said ETM is a metallocene.

53. A composition according to claim 52 wherein said metallocene is ferrocene.

54. A composition according to claim 53 wherein said ferrocene is a ferrocene derivative.

55. A composition according to claim 47 wherein said monolayer comprises conductive oligomers.

56. A composition according to claim 47 wherein said monolayer comprises insulators.

57. A composition according to claim 47 further comprising a target sequence, wherein said target sequence, said first nucleic acid, and said second nucleic acid form a hybridization complex.

58. A composition according to claim 57 wherein said target sequence comprises a first domain and a second domain, wherein said first nucleic acid hybridizes to said first domain and said second nucleic acid hybridizes to said second domain.