Reaction rate determination
First Claim
1. A method for determining the rate of a chemical reaction, comprising the following steps:
- (a) measuring signal data from either a sample or a reagent over a plurality of time points to obtain blank data;
(b) combining the reagent and the sample after step (a) to obtain a mixture, thereby initiating the chemical reaction;
(c) measuring signal data from the mixture over a plurality of time points to obtain reaction data; and
(d) determining a rate of reaction for the chemical reaction using the blank data and the reaction data.
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Abstract
One embodiment of the invention provides a method for determining the rate of reaction by including sample points before initiation of the reaction. Absorbance measurements are taken over a plurality of time points, prior to initiation of the reaction, to obtain zero-time absorbance points. The sample is combined with a reagent to obtain a mixture, thereby initiating the chemical reaction. Signal data measurements are then obtained for the mixture. The rate of reaction is then obtained for the mixture. For instance, the rate of reaction is obtained using a linear regression of the zero-time absorbance points and one or more reaction absorbance measurements. The reaction rate obtained may be correlated to known reaction rate data to determine the concentration of a chemical of interest in the sample.
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Citations
17 Claims
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1. A method for determining the rate of a chemical reaction, comprising the following steps:
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(a) measuring signal data from either a sample or a reagent over a plurality of time points to obtain blank data;
(b) combining the reagent and the sample after step (a) to obtain a mixture, thereby initiating the chemical reaction;
(c) measuring signal data from the mixture over a plurality of time points to obtain reaction data; and
(d) determining a rate of reaction for the chemical reaction using the blank data and the reaction data. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method of determining the concentration of an analyte of interest in a sample, comprising the following steps:
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(a) measuring signal data from either a sample or a reagent over a plurality of time points to obtain blank data;
(b) combining the reagent and the sample to form a mixture after step (a), thereby initiating a chemical reaction if the sample contains the analyte of interest;
(c) measuring signal data from the mixture over a plurality of time points to obtain reaction data;
(d) determining a rate of reaction for the chemical reaction using the blank data and the reaction data; and
(e) correlating the reaction rate to known reaction rate data to determine the concentration of the analyte of interest in the sample. - View Dependent Claims (12, 13, 14, 15, 16)
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17. A machine-readable medium having one or more instructions for determining the concentration of a chemical of interest in a sample, which when executed by a processor, causes the processor to perform operations comprising:
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(a) obtaining a liquid sample;
(b) measuring signal data from either a sample or a reagent over a plurality of time points to obtain blank data;
(c) combining the reagent and the sample to form a mixture after step (b), thereby initiating a chemical reaction;
(d) measuring signal data from the mixture over a plurality of time points to obtain reaction data;
(d) applying linear regression to the blank data and the measured reaction data to determine a rate of reaction for the chemical reaction; and
(e) correlating the reaction rate to known reaction rate data to determine the concentration of the first chemical of interest in the sample.
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Specification