Reaction rate determination

• US 20060205082A1
• Filed: 03/10/2005
• Published: 09/14/2006
• Est. Priority Date: 03/10/2005
• Status: Abandoned Application

• Current Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Original Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Inventors: Middleton, John S.
• Application Number:
  US11/078,670
• Publication Number:
  US 20060205082A1
• Time in Patent Office: Days
• US Class Current: 436/22
• CPC Class Codes: G01N 21/272 for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)

Abstract

One embodiment of the invention provides a method for determining the rate of reaction by including sample points before initiation of the reaction. Absorbance measurements are taken over a plurality of time points, prior to initiation of the reaction, to obtain zero-time absorbance points. The sample is combined with a reagent to obtain a mixture, thereby initiating the chemical reaction. Signal data measurements are then obtained for the mixture. The rate of reaction is then obtained for the mixture. For instance, the rate of reaction is obtained using a linear regression of the zero-time absorbance points and one or more reaction absorbance measurements. The reaction rate obtained may be correlated to known reaction rate data to determine the concentration of a chemical of interest in the sample.

Claims

1. A method for determining the rate of a chemical reaction, comprising the following steps:

(a) measuring signal data from either a sample or a reagent over a plurality of time points to obtain blank data;

(b) combining the reagent and the sample after step (a) to obtain a mixture, thereby initiating the chemical reaction;

(c) measuring signal data from the mixture over a plurality of time points to obtain reaction data; and

(d) determining a rate of reaction for the chemical reaction using the blank data and the reaction data.

2. The method of claim 1, wherein the steps of measuring signal data from either the sample or the reagent and measuring signal data from the mixture include measuring optical signal data.

3. The method of claim 1, wherein the steps of measuring signal data from either the sample or the reagent and measuring signal data from the mixture include measuring voltage data.

4. The method of claim 1, wherein the steps of measuring signal data from either the sample or the reagent and measuring signal data from the mixture include measuring conductivity data.

5. The method of claim 1, wherein the steps of measuring signal data from either the sample or the reagent and measuring signal data from the mixture include measuring temperature data.

6. The method of claim 1 further comprising:

correlating the reaction rate to known reaction rate data to determine a concentration of a chemical of interest in the sample.

7. The method of claim 1 wherein the blank data includes blank absorbance data, further comprising:

determining a corrected absorbance data for either the sample or the reagent that compensates for sample dilution.

8. The method of claim 1 further comprising:

obtaining the sample, wherein the sample is a liquid sample.

9. The method of claim 1 further comprising:

obtaining the reagent, wherein the reagent is a liquid reagent.

10. The method of claim 1 further comprising:

applying linear regression to the blank data and the reaction data to determine the rate of reaction for the chemical reaction.

11. A method of determining the concentration of an analyte of interest in a sample, comprising the following steps:

(a) measuring signal data from either a sample or a reagent over a plurality of time points to obtain blank data;

(b) combining the reagent and the sample to form a mixture after step (a), thereby initiating a chemical reaction if the sample contains the analyte of interest;

(c) measuring signal data from the mixture over a plurality of time points to obtain reaction data;

(d) determining a rate of reaction for the chemical reaction using the blank data and the reaction data; and

(e) correlating the reaction rate to known reaction rate data to determine the concentration of the analyte of interest in the sample.

12. The method of claim 11 further comprising:

obtaining the sample to be tested for the analyte of interest, wherein the sample is a liquid sample; and

obtaining the reagent, wherein the reagent is a liquid reagent.

13. The method of claim 11, wherein the steps of measuring signal data from either the sample or the reagent and measuring signal data from the mixture include at least one step of

measuring optical signal data;

measuring voltage data;

measuring conductivity data;

or

measuring temperature data.

14. The method of claim 11, further comprising:

determining a corrected blank signal data for either the sample or the reagent that compensates for sample dilution.

15. The method of claim 11 further comprising:

applying linear regression to the blank data and the reaction data to determine the rate of reaction for the chemical reaction.

16. The method of claim 11 wherein the linear regression is weighted by the plurality blank data points.

17. A machine-readable medium having one or more instructions for determining the concentration of a chemical of interest in a sample, which when executed by a processor, causes the processor to perform operations comprising:

(a) obtaining a liquid sample;

(b) measuring signal data from either a sample or a reagent over a plurality of time points to obtain blank data;

(c) combining the reagent and the sample to form a mixture after step (b), thereby initiating a chemical reaction;

(d) measuring signal data from the mixture over a plurality of time points to obtain reaction data;

(d) applying linear regression to the blank data and the measured reaction data to determine a rate of reaction for the chemical reaction; and

(e) correlating the reaction rate to known reaction rate data to determine the concentration of the first chemical of interest in the sample.