Method and apparatus for glucose monitoring
First Claim
1. A device for sensing an analyte, said device comprising:
- a ligand selected from the group consisting of dextran, a functionalized polymer, and a functionalized nano-particle;
a first fluorophore selected from the group consisting of a fluoresceine, a rhodamine, a cyanine, an alexafluore, and combinations thereof;
a sugar-binding lectin selected from the group consisting of Concanavalin A, glucose binding protein, glucose oxidase, glucose apo-oxidase, and combinations thereof; and
a second fluorophore selected from the group consisting of a fluoresceine, a rhodamine, a cyanine, an alexafluore, and combinations thereof, wherein the first fluorophore is linked to the ligand and the second fluorophore is linked to the sugar-binding lectin and wherein the first fluorophore is a reference fluorophore and the second fluorophore is quenchable fluorophore and the second fluorophore is a reference fluorophore.
4 Assignments
0 Petitions
Accused Products
Abstract
This disclosure relates to systems, devices, and methods of sensing an analyte. An implantable sensor may be contacted with a test sample under conditions that permit a binding protein and a ligand of the sensor to interact in an analyte-dependent manner to produce an analyte-dependent signal, and (b) detecting the analyte-dependent signal with a detector. A binding protein may reversibly bind an analyte and/or a ligand. A binding protein may have a higher binding affinity for an analyte than for a ligand. A binding protein and a ligand may each include a fluorophore, the absorption and/or emission properties of which may change in an analyte-dependent manner. A binding protein and/or a ligand may be bound to an active or inactive substrate. Some embodiments of systems, devices, and methods may be practiced in vitro, in situ, and/or in vivo. Systems and/or devices of the disclosure may be configured to be wearable.
-
Citations
21 Claims
-
1. A device for sensing an analyte, said device comprising:
-
a ligand selected from the group consisting of dextran, a functionalized polymer, and a functionalized nano-particle;
a first fluorophore selected from the group consisting of a fluoresceine, a rhodamine, a cyanine, an alexafluore, and combinations thereof;
a sugar-binding lectin selected from the group consisting of Concanavalin A, glucose binding protein, glucose oxidase, glucose apo-oxidase, and combinations thereof; and
a second fluorophore selected from the group consisting of a fluoresceine, a rhodamine, a cyanine, an alexafluore, and combinations thereof, wherein the first fluorophore is linked to the ligand and the second fluorophore is linked to the sugar-binding lectin and wherein the first fluorophore is a reference fluorophore and the second fluorophore is quenchable fluorophore and the second fluorophore is a reference fluorophore. - View Dependent Claims (2, 3, 4, 5, 6)
-
-
7. A system for sensing glucose, said system comprising:
-
(a) an implantable glucose-sensing device comprising;
a hydrogel;
a ligand selected from the group consisting of dextran, a functionalized polymer, and a functionalized nano-particle;
a first fluorophore selected from the group consisting of a fluoresceine, a rhodamine, a cyanine, an alexafluore, and combinations thereof;
a sugar-binding lectin selected from the group consisting of Concanavalin A, glucose binding protein, glucose oxidase, glucose apo-oxidase, and combinations thereof; and
a second fluorophore selected from the group consisting of a fluoresceine, a rhodamine, a cyanine, an alexafluore, and combinations thereof, wherein the ligand and the sugar-binding lectin are present throughout the matrix of the hydrogel, wherein the first fluorophore is linked to the ligand and the second fluorophore is linked to the sugar-binding lectin, wherein the first fluorophore is a fluorescence resonance energy transfer donor or fluorescence resonance energy transfer acceptor and the second fluorophore is a fluorescence resonance energy transfer donor or fluorescence resonance energy transfer acceptor provided that at least one of the fluorophores is a fluorescence resonance energy transfer donor and at least one of the fluorophores is a fluorescence resonance energy transfer acceptor; and
(b) a light source configured and arranged to illuminate the implantable glucose sensing device with light of a wavelength sufficient to excite the fluorescence resonance energy transfer donor;
(c) a detector (a) in optical communication with the implantable glucose sensing device and (b) configured and arranged to detect at least at least one wavelength of light from the implantable glucose sensing device and produce a detected light signal;
(d) a processor in communication with the detector and configured and arranged to receive and process at least one detected light signal to produce a glucose metric;
(e) a display operably linked to the processor and configured and arranged to receive and display the glucose metric; and
(f) a power source operably linked to at least the light source, the detector, the processor, and the display. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
-
-
20. A method of sensing glucose, said method comprising:
-
contacting an implantable glucose sensor with a test sample under conditions that permit the binding protein and ligand of the sensor to interact in a glucose dependent manner to produce a glucose-dependent signal;
detecting the glucose-dependent signal with a detector; and
processing a glucose-dependent signal to produce a glucose metric, wherein
-
-
21. A device for sensing an analyte, said device comprising:
-
a ligand selected from the group consisting of dextran, a functionalized polymer, and a functionalized nano-particle;
a first fluorophore selected from the group consisting of a fluoresceine, a rhodamine, a cyanine, an alexafluore, and combinations thereof;
a sugar-binding lectin selected from the group consisting of Concanavalin A, glucose binding protein, glucose oxidase, glucose apo-oxidase, and combinations thereof; and
a second fluorophore selected from the group consisting of a fluoresceine, a rhodamine, a cyanine, an alexafluore, and combinations thereof, wherein the first fluorophore is linked to the ligand and the second fluorophore is linked to the sugar-binding lectin and wherein the first fluorophore is a fluorescence resonance energy transfer donor or fluorescence resonance energy transfer acceptor and the second fluorophore is a fluorescence resonance energy transfer donor or fluorescence resonance energy transfer acceptor provided that at least one of the fluorophores is a fluorescence resonance energy transfer donor and at least one of the fluorophores is a fluorescence resonance energy transfer acceptor.
-
Specification