Biosensor and its use

• US 20070161069A1
• Filed: 11/14/2006
• Published: 07/12/2007
• Est. Priority Date: 11/17/2005
• Status: Active Grant

• Current Assignee: École centrale de Lyon, Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forsching E.V., Institute of Catalysis
• Original Assignee: École centrale de Lyon, Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forsching E.V., Institute of Catalysis
• Inventors: Betancor, Lorena, Rose, Klaus, Jaffrezic, Nicole, Dzyadevych, Sergei, Guisan, Jose, Lafuente, Roberto
• Granted Patent: US 7,709,221 B2
• Time in Patent Office: Days
• US Class Current: 435/14
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

The present invention relates to a biosensor comprising a substrate with a coating system in which a Ruthenium complex and an enzyme is integrated. The enzyme is able to convert bioproducts, e.g. glucose, fructose or glycerol. The depletion of oxygen during these converting reactions can be monitored via the fluorescence of the Ruthenium complex. The inventive biosensor can be used in biotechnological processes, e.g. the synthesis of biofuels.

Claims

1. A biosensor comprising a substrate with at least one coating of an inorganic-organic hybrid polymer as a matrix for at least one Ruthenium complex and at least one enzyme for converting bioproducts.

2. The biosensor of claim 1, wherein the substrate is a glass and/or a polymer.

3. The biosensor of claim 1, wherein the substrate is an optical lens or an optical fibre.

4. The biosensor of claim 1, wherein the substrate is an optical fibre having a cladding layer.

5. The biosensor of claim 1, wherein the Ruthenium complex is selected from the group consisting of complexes of

6. The biosensor of claim 1, wherein the enzyme is selected from the group consisting of glucose oxidase from Penicillinium vitale, glucose oxidase from Aspergillus niger, glucose oxidase from Aspergillus niger type II and glucose oxidase from Aspergillus niger type IIS for determining glucose.

7. The biosensor of claim 1, wherein the enzyme is selected from the group consisting of glucose oxidase, glucose isomerase and glucose catalase for determining fructose.

8. The biosensor of claim 1, wherein the enzyme is selected from the group consisting of glycerokinase, L-Glycerol-3-phosphate and catalase for determining glycerol.

9. The biosensor of claim 1, wherein the enzyme is immobilized in a polymeric host matrix.

10. The biosensor of claim 1, wherein the polymeric host matrix is selected from the group polystyrene, styrene/divinylbenzene copolymer and polyacrylics.

11. The biosensor of claim 1, wherein the inorganic-organic hybrid polymer is obtained by hydrolytic condensation of at least one alkoxysilane.

12. The biosensor of claim 1, wherein the inorganic-organic hybrid polymer is obtained by hydrolytic condensation of at least one organically substituted alkoxysilane and at least one alkoxy compound of a metal.

13. The biosensor of claim 1, wherein the alkoxy compound of a metal is selected from the group consisting of Si(OEt)₄, Ti(OEt)₄, Zr(OPr)₄ and Al(O^sBu)₃.

14. The biosensor of claim 1, wherein the substrate has a single coating comprising the at least one Ruthenium complex and the at least one enzyme.

15. The biosensor of claim 1, wherein the substrate has a first coating containing the at least one Ruthenium complex and a second coating containing the at least one enzyme.

16-18. -18. (canceled)

19. A method of measuring depletion of oxygen in a fluid comprising contacting the fluid with a biosensor comprising a substrate with at least one coating of an inorganic-organic hybrid polymer as a matrix for at least one Ruthenium complex and at least one enzyme for converting bioproducts.

20. The method of claim 19, wherein the fluid is a fluid of a biotechnical process.

21. The method of claim 19, wherein the fluid is a fluid of the food and drink industry.

22. The method of claim 19, wherein the fluid is a fluid of pharmaceutical or waste processing or of the process of synthesizing biofuel.