Un-supported Polymeric Film with Embedded Microbeads

• US 20070248993A1
• Filed: 06/12/2007
• Published: 10/25/2007
• Est. Priority Date: 06/21/2001
• Status: Active Grant

• Current Assignee: BioArray Solutions Limited (Werfen SA)
• Original Assignee: BioArray Solutions Limited (Werfen SA)
• Inventors: Seul, Michael, Banerjee, Sukanta, Hong, Ye, Podual, Kairali
• Granted Patent: US 9,436,088 B2
• Time in Patent Office: Days
• US Class Current: 435/7.100
• CPC Class Codes: C08J 2333/26 Homopolymers or copolymers of acrylamide or methacrylamide
• CPC Class Codes: C08J 2425/06 Polystyrene
• CPC Class Codes: C08J 3/075 Macromolecular gels
• CPC Class Codes: C08J 3/28 Treatment by wave energy or particle radiation
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025
• CPC Class Codes: G01N 33/545 Synthetic resin
• CPC Class Codes: G03F 7/0047 characterised by additives for obtaining a metallic or ceramic pattern, e.g. by firing
• CPC Class Codes: G03F 7/164 using electric, electrostatic or magnetic means; powder coating
• CPC Class Codes: Y10T 428/249952 At least one thermosetting synthetic polymeric material layer
• CPC Class Codes: Y10T 428/25 Web or sheet containing structurally defined element or component and including a second component containing structurally defined particles

Abstract

The present invention relates to a systematic process for the creation of functionally organized, spatially patterned assemblies polymer-microparticle composites including the AC electric field-mediated assembly of patterned, self supporting organic (polymeric) films and organic (polymeric)—microparticle composite films of tailored composition and morphology; the present invention further relates to the incorporation of said assemblies into other structures. The present invention also relates to the application of such functional assemblies in materials science and biology. Additional areas of application include sensors, catalysts, membranes, micro-reactors, smart materials. Miniaturized format for generation of multifunctional thin films. Provides a simple set-up to synthesize thin films of tailored composition and morphology:

Claims

1. A polymer-bead composite comprising:

a single layer planar assembly of encoded beads embedded in a hydrophilic polymeric matrix and not attached to a solid support, wherein differently—

encoded beads have different biomolecules attached to their surfaces.

2. The polymer-bead composite of claim 1 wherein the particles are encoded with a unique chemical or physical characteristic.

3. The polymer-bead composite of claim 2 wherein the encoding is with a fluorescent dye.

4. The polymer particle composite of claim 3 wherein the biomolecules are peptides, proteins, nucleic acids, oligonucleotide, ligands or receptors.

5. The polymer particle composite of claim 1 wherein the polymer is alkylacrylamide or hydroxyacrylalkylate hydrogel.

6. The polymer particle composite of claim 1 wherein the beads are magnetic beads.