Analysis kit for living organism and chemical reaction

• US 20070292897A1
• Filed: 06/15/2007
• Published: 12/20/2007
• Est. Priority Date: 06/19/2006
• Status: Active Grant

• Current Assignee: Hitachi, Ltd.
• Original Assignee: Hitachi, Ltd.
• Inventors: Yazawa, Yoshiaki, Uchida, Kenko, Anazawa, Takashi
• Granted Patent: US 7,985,384 B2
• Time in Patent Office: Days
• US Class Current: 435/7.9
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: G01N 33/54388 based on lateral flow
• CPC Class Codes: G01N 33/558 using diffusion or migration of antigen or antibody immunochromatographic test strips G01N33/54387

Abstract

There is provided a simple, inexpensive reaction analysis kit and system capable of performing highly sensitive, quantitative measurement. A chemical luminescence reaction is employed and a sensor element is used to detect the reaction in a highly sensitive manner. That is, a reaction detection plate is used to transmit a signal detected in the sensor element via a reader coil and a reader and then analyze the reaction. The reaction detection plate has a) a membrane, b) a first antibody impregnated section that is disposed such that it faces the membrane and holds a first labeled antibody that specifically binds to a substance to be analyzed, c) a second antibody immobilized section that is provided in part of the membrane and has an immobilized second antibody, the second antibody specifically binding to the substance to be analyzed, and d) a sensor element that is disposed such that it faces the second antibody immobilized section and includes a light detector and a signal transceiver.

Claims

1. A reaction analysis kit comprising:

a first labeled antibody that specifically binds to a substance to be analyzed, a reagent that reacts with the labeling substance and emits light, and a reaction detection plate,

wherein the reaction detection plate includes a) a membrane, b) a first antibody impregnated section that is disposed such that it faces the membrane and holds the first antibody, c) a second antibody immobilized section that is disposed in part of the membrane and has an immobilized second antibody, the second antibody specifically binding to the substance to be analyzed, and d) a sensor element that is disposed such that it faces the second antibody immobilized section and includes a light detector and a signal transceiver.

2. The reaction analysis kit according to claim 1, wherein the reaction detection plate is housed in a shielding container.

3. The reaction analysis kit according to claim 1, wherein the second antibody immobilized section is substantially circular.

4. The reaction analysis kit according to claim 1, wherein the center of the light detector faces the center of the second antibody immobilized section.

5. The reaction analysis kit according to claim 1, wherein the sensor element is disposed on the same side as the first antibody impregnated section with respect to the membrane.

6. The reaction analysis kit according to claim 1, wherein the sensor element is disposed on the opposite side of the first antibody impregnated section to the membrane.

7. The reaction analysis kit according to claim 1, wherein the region of the second antibody immobilized section that does not face the light detector has undergone an antibody inactivation process.

8. The reaction analysis kit according to claim 1, wherein the center of the light detector faces the center of the second antibody immobilized section, the diameter of the light detector is 10 μ

m to 2 mm, and a region apart from the center of the second antibody immobilized section by 2.5 mm or greater has undergone an antibody inactivation process.

9. The reaction analysis kit according to claim 1 further comprising a projection that fills the gap between the membrane and the shielding container or a light-blocking filter in the shielding container in order to block light from entering the sensor element.

10. The reaction analysis kit according to claim 1, wherein the number of the sensor element is at least two.

11. The reaction analysis kit according to claim 1, wherein for two or more substances to be analyzed, a plurality of second antibody immobilized regions and a plurality of sensor elements corresponding to the respective substances to be analyzed are provided.

12. The reaction analysis kit according to claim 1 further comprising a mirror disposed such that it covers part or all of the second antibody immobilized section.

13. The reaction analysis kit according to claim 1, wherein the shielding container has a guiding structure for defining the position where the sensor element is disposed.

14. The reaction analysis kit according to claim 1, wherein part of the membrane has a light absorbing region for preventing crosstalk.

15. A reaction analysis system comprising:

a reaction detection plate including a) a membrane, b) a first antibody impregnated section that is disposed such that it faces the membrane and holds a first labeled antibody that specifically binds to a substance to be analyzed, c) a second antibody immobilized section that is disposed in part of the membrane and has an immobilized second antibody, the second antibody specifically binding to the substance to be analyzed, and d) a sensor element that is disposed such that it faces the second antibody immobilized section and includes a light detector and a signal transceiver;

a shielding container that houses the reaction detection plate;

a reader coil that is disposed outside the shielding container, the reader coil transmitting and receiving a signal to and from the sensor element and supplying power to the sensor element;

a reader that performs demodulation/modulation, decoding/coding and amplification of the signal; and

a processor for executing an application program that controls the sensor element.

16. The reaction analysis system according to claim 15 further comprising a sensor element-side coil in the shielding container.

17. The reaction analysis system according to claim 16 further comprising a relay coil that inductively couples the sensor element-side coil to the reader coil.

18. The reaction analysis system according to claim 15, wherein the number of the sensor element is at least two.