Apparatus and method for cell migration assays

• US 20080032324A1
• Filed: 03/28/2007
• Published: 02/07/2008
• Est. Priority Date: 09/28/2004
• Status: Abandoned Application

• Current Assignee: Trustees of Tufts College
• Original Assignee: Trustees of Tufts College
• Inventors: Walt, David, Di Cesare, Christopher
• Application Number:
  US11/729,431
• Publication Number:
  US 20080032324A1
• Time in Patent Office: Days
• US Class Current: 435/29
• CPC Class Codes: G01N 15/01 specially adapted for biological cells, e.g. blood cells investigating sedimentation of particle suspensions in blood G01N15/05
• CPC Class Codes: G01N 15/1433 using image recognition
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6458 Fluorescence microscopy fluorescence microscopes per se G02B21/0076 and G02B21/16

Abstract

The present invention is directed to a method and apparatus for detecting and analyzing cell migration. More specifically, the present invention is directed to novel technology for analyzing cellular movement, including whole cell migration and subcellular component movement. Cells are distributed onto a substrate and monitored for migration or movement. According to one embodiment, when a labelled cell or portion of a cell passes over one of the delineations between detection units, such as individual fibers in a fiber optic bundle, the label causes a large intensity increase, which stays for a given “residence time” until the cell departs from the detection unit.

Claims

1. A method of detecting cell migration, the method comprising:

providing a plurality of cells on a substrate comprising a plurality of detection units, wherein each of said cells is disposed over at least one of said detection units; and

detecting cellular movement of at least one of said cells.

2. The method of claim 1, wherein each of said cells is disposed over more than one of said detection units.

3. The method of claim 1, further comprising adding an analyte of interest prior to said detecting cellular movement.

4. The method of claim 1, further comprising:

adding an analyte of interest after said detecting cellular movement; and

detecting cellular movement of at least one of said cells a second time after adding said analyte.

5. The method of claim 4, further comprising comparing data from said detecting cellular movement steps to identify any effect of said analyte of interest on cellular movement.

6. The method of claim 5, further comprising comparing data from both of said detecting cellular movement steps to measure any effect of said analyte of interest on cellular movement.

7. The method of claim 1, wherein said detecting cellular movement comprises detecting movement of at least one of said cells in relation to at least one of said detection units.

8. The method of claim 1, wherein said detecting cellular movement comprises detecting movement of a subcellular component of at least one of said cells in relation to at least one of said detection units.

9. The method of claim 1, wherein said detecting cellular movement comprises detecting residence time in relation to at least one of said detection units.

10. The method of claim 9, wherein said residence time is an amount of time during which at least a portion of at least one of said cells is disposed above at least one of said detection units.

11. The method of claim 1, wherein at least one of said plurality of cells comprises a label.

12. The method of claim 11, wherein the label is a fluorescent label.

13. The method of claim 11, wherein a subcellular component of said at least one of said plurality of cells comprises the label.

14. The method of claim 11, wherein said detecting cellular movement comprises detecting movement of at least one of said labels in relation to at least one of said detection units.

15. The method of claim 11, wherein said detecting cellular movement comprises detecting residence time of at least one of said labels in relation to at least one of said detection units.

16. The method of claim 15, wherein said residence time is an amount of time during which at least a portion of at least one of said labels is disposed above at least one of said detection units.

17. The method of claim 1, wherein said providing a plurality of cells comprises providing at least two cell types on said substrate.

18. The method of claim 1, wherein each of said plurality of cells has a diameter greater than a diameter of any of said plurality of detection units.

19. The method of claim 1, further comprising testing viability of said cells.

20. A composition for detection of cellular migration, the composition comprising:

a substrate comprising a plurality of detection units; and

a plurality of cells distributed on said substrate such that each of said cells is disposed over at least one of said detection units.

21. The composition of claim 20, wherein each of said cells is disposed over more than one of said detection units.

22. The composition of claim 20, further comprising a detection component.

23. The composition of claim 22, wherein the detection component comprises a microscope.

24. The composition of claim 20, wherein said substrate comprises a cell adhesion facilitator.

25. The composition of claim 22, wherein the composition is a biosensor array.

26. The composition of claim 20, wherein said substrate is at least one fiber optic bundle.

27. The composition of claim 26, wherein said detection units are fiber strands.

28. The composition of claim 20, wherein each of said detection units is a discrete optically viewable site in said substrate.

29. The composition of claim 20, wherein said substrate is selected from the group consisting of plastic and glass.

30. The composition of claim 20, further comprising an analyte of interest distributed on said substrate.

31. The composition of claim 20, wherein at least one of said plurality of cells comprises a label.

32. The composition of claim 31, wherein the label is a fluorescent label.

33. The composition of claim 20, wherein said plurality of cells comprises at least two cell types.

34. The composition of claim 20, wherein each of said plurality of cells has a diameter greater than a diameter of any of said plurality of detection units.

35. The composition of claim 20, wherein said detection units have a density ranging from about _—

to about _—

36. The composition of claim 20, where said detection units have a size ranging from about _—

to about _.

37. The composition of claim 20, further comprising a temperature regulator.

38. The composition of claim 20, further comprising a computer.

39. The composition of claim 38, wherein said computer is configured to analyze a signal from said substrate.